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rabbit polyclonal anti histone modifications  (Active Motif)


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    Structured Review

    Active Motif rabbit polyclonal anti histone modifications
    Rabbit Polyclonal Anti Histone Modifications, supplied by Active Motif, used in various techniques. Bioz Stars score: 97/100, based on 960 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/histone+modification+antibodies/Histone+H3K4me3+antibody+(pAb)/pm40318218-71-67-74
    Average 97 stars, based on 960 article reviews
    rabbit polyclonal anti histone modifications - by Bioz Stars, 2026-09
    97/100 stars

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    Incubation:

    Article Title: Rescuing DNMT1 fails to fully reverse the molecular and functional repercussions of its loss in mouse embryonic stem cells
    Article Snippet: .. Precleared chromatin samples were incubated overnight at 4°C on a rotator with histone modification antibodies—either anti-H3K4me3 (Active Motif, 39159), anti-H3K27ac (Abcam, ab4729), anti-H3K4me1 (Cell Signaling Technology, 5326), anti-H3K27me3 (Sigma–Aldrich, 07-449), or anti-H3K9me3 (Abcam, ab8898)—in quantities recommended by the antibody manufacturer. ..

    Article Title: Rescuing DNMT1 fails to fully reverse the molecular and functional repercussions of its loss in mouse embryonic stem cells.
    Article Snippet: .. Precleared chromatin samples were incubated overnight at 4 ◦C on a rotator with histone modification antibodies—either anti-H3K4me3 (Active Motif, 39159), anti-H3K27ac (Abcam, ab4729), anti-H3K4me1 (Cell Signaling Technology, 5326), anti-H3K27me3 (Sigma–Aldrich, 07-449), or anti-H3K9me3 (Abcam, ab8898)—in quantities recommended by the antibody manufacturer. ..

    Modification:

    Article Title: Rescuing DNMT1 fails to fully reverse the molecular and functional repercussions of its loss in mouse embryonic stem cells
    Article Snippet: .. Precleared chromatin samples were incubated overnight at 4°C on a rotator with histone modification antibodies—either anti-H3K4me3 (Active Motif, 39159), anti-H3K27ac (Abcam, ab4729), anti-H3K4me1 (Cell Signaling Technology, 5326), anti-H3K27me3 (Sigma–Aldrich, 07-449), or anti-H3K9me3 (Abcam, ab8898)—in quantities recommended by the antibody manufacturer. ..

    Article Title: The K219T-Lamin mutation induces conduction defects through epigenetic inhibition of SCN5A in human cardiac laminopathy
    Article Snippet: Fixed cells were lysed in IP buffer made from two volumes of SDS buffer (100mM NaCl, 50mM Tris-HCl pH 8.1, 5mM EDTA pH 8.0, 0.5% SDS), one volume of Triton buffer (100mM NaCl, 100mM Tris-HCl pH 8.1, 5mM EDTA pH 8, 5% Triton X-100) and protease inhibitors (Roche) for 10 min on ice. .. Chromatin was sonicated with a Bioruptor Diagenode Sonicator and immunoprecipitated overnight on a wheel at 4°C with 4μg Lamin A/C antibody (anti Lamin A/C (636), from Santa Cruz Biotechnology, sc-7292 x), 5μg histone modification antibodies (anti-H3K4me3, from Active Motif, 39159; anti-H3K27me3, from Abcam, ab6002; anti-H3K9 me3, from Abcam, ab8898) and anti-Suz12 antibodies (Cell Signaling, #3737) at 1:100. ..

    Article Title: Sin3a acts through a multi-gene module to regulate invasion in Drosophila and human tumors
    Article Snippet: Anti-Sin3A, anti-Sin3B, anti-Actin, anti-GAPDH, anti-PP1α and anti-RhoA antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA) and anti-β-Catenin antibody (from BD Biosciences, San Jose, CA, USA). .. All histone modification antibodies were obtained from ActiveMotif (Carlsbad, CA, USA). ..

    Article Title: Rescuing DNMT1 fails to fully reverse the molecular and functional repercussions of its loss in mouse embryonic stem cells.
    Article Snippet: .. Precleared chromatin samples were incubated overnight at 4 ◦C on a rotator with histone modification antibodies—either anti-H3K4me3 (Active Motif, 39159), anti-H3K27ac (Abcam, ab4729), anti-H3K4me1 (Cell Signaling Technology, 5326), anti-H3K27me3 (Sigma–Aldrich, 07-449), or anti-H3K9me3 (Abcam, ab8898)—in quantities recommended by the antibody manufacturer. ..

    Sonication:

    Article Title: The K219T-Lamin mutation induces conduction defects through epigenetic inhibition of SCN5A in human cardiac laminopathy
    Article Snippet: Fixed cells were lysed in IP buffer made from two volumes of SDS buffer (100mM NaCl, 50mM Tris-HCl pH 8.1, 5mM EDTA pH 8.0, 0.5% SDS), one volume of Triton buffer (100mM NaCl, 100mM Tris-HCl pH 8.1, 5mM EDTA pH 8, 5% Triton X-100) and protease inhibitors (Roche) for 10 min on ice. .. Chromatin was sonicated with a Bioruptor Diagenode Sonicator and immunoprecipitated overnight on a wheel at 4°C with 4μg Lamin A/C antibody (anti Lamin A/C (636), from Santa Cruz Biotechnology, sc-7292 x), 5μg histone modification antibodies (anti-H3K4me3, from Active Motif, 39159; anti-H3K27me3, from Abcam, ab6002; anti-H3K9 me3, from Abcam, ab8898) and anti-Suz12 antibodies (Cell Signaling, #3737) at 1:100. ..

    Immunoprecipitation:

    Article Title: The K219T-Lamin mutation induces conduction defects through epigenetic inhibition of SCN5A in human cardiac laminopathy
    Article Snippet: Fixed cells were lysed in IP buffer made from two volumes of SDS buffer (100mM NaCl, 50mM Tris-HCl pH 8.1, 5mM EDTA pH 8.0, 0.5% SDS), one volume of Triton buffer (100mM NaCl, 100mM Tris-HCl pH 8.1, 5mM EDTA pH 8, 5% Triton X-100) and protease inhibitors (Roche) for 10 min on ice. .. Chromatin was sonicated with a Bioruptor Diagenode Sonicator and immunoprecipitated overnight on a wheel at 4°C with 4μg Lamin A/C antibody (anti Lamin A/C (636), from Santa Cruz Biotechnology, sc-7292 x), 5μg histone modification antibodies (anti-H3K4me3, from Active Motif, 39159; anti-H3K27me3, from Abcam, ab6002; anti-H3K9 me3, from Abcam, ab8898) and anti-Suz12 antibodies (Cell Signaling, #3737) at 1:100. ..



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